##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	stdin
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1000000
Total Bases	150 Mbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.109128	40.0	40.0	40.0	40.0	40.0
2	39.203456	40.0	40.0	40.0	40.0	40.0
3	39.28116	40.0	40.0	40.0	40.0	40.0
4	39.424349	40.0	40.0	40.0	40.0	40.0
5	39.51487	40.0	40.0	40.0	40.0	40.0
6	39.591518	40.0	40.0	40.0	40.0	40.0
7	39.444454	40.0	40.0	40.0	40.0	40.0
8	39.490107	40.0	40.0	40.0	40.0	40.0
9	39.446207	40.0	40.0	40.0	40.0	40.0
10-14	39.480293599999996	40.0	40.0	40.0	40.0	40.0
15-19	39.571991	40.0	40.0	40.0	40.0	40.0
20-24	39.612361799999995	40.0	40.0	40.0	40.0	40.0
25-29	39.5709978	40.0	40.0	40.0	40.0	40.0
30-34	39.4706852	40.0	40.0	40.0	40.0	40.0
35-39	39.429067200000006	40.0	40.0	40.0	40.0	40.0
40-44	39.3932734	40.0	40.0	40.0	40.0	40.0
45-49	39.324989	40.0	40.0	40.0	40.0	40.0
50-54	39.2718552	40.0	40.0	40.0	40.0	40.0
55-59	39.2346702	40.0	40.0	40.0	40.0	40.0
60-64	39.1475516	40.0	40.0	40.0	40.0	40.0
65-69	39.115598	40.0	40.0	40.0	40.0	40.0
70-74	39.0497574	40.0	40.0	40.0	40.0	40.0
75-79	38.9525934	40.0	40.0	40.0	40.0	40.0
80-84	38.8736334	40.0	40.0	40.0	40.0	40.0
85-89	38.776672000000005	40.0	40.0	40.0	40.0	40.0
90-94	38.655975399999996	40.0	40.0	40.0	40.0	40.0
95-99	38.545458599999996	40.0	40.0	40.0	40.0	40.0
100-104	38.4198168	40.0	40.0	40.0	40.0	40.0
105-109	38.2768942	40.0	40.0	40.0	40.0	40.0
110-114	38.0931462	40.0	40.0	40.0	40.0	40.0
115-119	37.8966816	40.0	40.0	40.0	36.8	40.0
120-124	37.561758600000005	40.0	40.0	40.0	24.0	40.0
125-129	37.21200879999999	40.0	40.0	40.0	24.0	40.0
130-134	36.751611600000004	40.0	40.0	40.0	24.0	40.0
135-139	36.4125186	40.0	40.0	40.0	24.0	40.0
140-144	36.11292160000001	40.0	40.0	40.0	24.0	40.0
145-149	35.7968054	40.0	40.0	40.0	24.0	40.0
150	35.619862	40.0	40.0	40.0	24.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1102	1	0.0743213682641155
1102	2	0.03213559047176773
1102	3	0.12987579629391632
1102	4	-0.07198433214406208
1102	5	-0.1168702495822771
1102	6	-0.10469886124171524
1102	7	-0.10108021448976956
1102	8	-0.16414331061534426
1102	9	-0.12401846307719211
1102	10-14	-0.12329569091143355
1102	15-19	-0.09456869572451865
1102	20-24	-0.06075872666596638
1102	25-29	-0.04859382814679947
1102	30-34	-0.10919300490877504
1102	35-39	-0.10258504761095111
1102	40-44	-0.08480016362447174
1102	45-49	-0.12414864775014678
1102	50-54	-0.10581905776158607
1102	55-59	-0.09552026771854827
1102	60-64	-0.0676820693323279
1102	65-69	-0.06981147467379856
1102	70-74	-0.09188656798221473
1102	75-79	-0.07914134004652595
1102	80-84	-0.1198129559701897
1102	85-89	-0.12204233082366756
1102	90-94	-0.13053491307681497
1102	95-99	-0.12883096920849368
1102	100-104	-0.12751604019162244
1102	105-109	-0.14910202644983883
1102	110-114	-0.1834855284748258
1102	115-119	-0.1935574031394296
1102	120-124	-0.2585963214268574
1102	125-129	-0.21786199886409463
1102	130-134	-0.22433926913585367
1102	135-139	-0.30931013635370874
1102	140-144	-0.3082755544590796
1102	145-149	-0.23059811704320765
1102	150	-0.23077399686967226
1103	1	0.030666766771012988
1103	2	0.05415489791103312
1103	3	-0.0600436461497722
1103	4	-0.022750868527730006
1103	5	-0.0959305562057935
1103	6	-0.06444805305965673
1103	7	-0.07889505048957801
1103	8	-0.1035575969693241
1103	9	-0.13270231493154228
1103	10-14	-0.1669251552877853
1103	15-19	-0.09686567775995769
1103	20-24	-0.0828952892522139
1103	25-29	-0.06268529775775988
1103	30-34	-0.04024364360356003
1103	35-39	-0.025927152788000285
1103	40-44	-0.0498834265136594
1103	45-49	-0.0752918589872209
1103	50-54	-0.09329966379021215
1103	55-59	-0.08012665422260312
1103	60-64	-0.0594666069485541
1103	65-69	-0.10110271340698773
1103	70-74	-0.08823290240744086
1103	75-79	-0.0902923156405322
1103	80-84	-0.09050011231548183
1103	85-89	-0.10273151364728506
1103	90-94	-0.12050248538938746
1103	95-99	-0.1254829204121819
1103	100-104	-0.13972947313870065
1103	105-109	-0.1326278324926804
1103	110-114	-0.1562510492436715
1103	115-119	-0.12328407957735976
1103	120-124	-0.19445232339980834
1103	125-129	-0.16636076928346455
1103	130-134	-0.1391648707513653
1103	135-139	-0.26463192528986923
1103	140-144	-0.3318932741362133
1103	145-149	-0.26827705393680645
1103	150	-0.4823513262921679
1104	1	-0.02594792785663458
1104	2	-0.004815101708295799
1104	3	-0.09628648045580235
1104	4	-0.14824114244970588
1104	5	-0.0923789344652377
1104	6	-0.05255148223805861
1104	7	-0.14425258994982215
1104	8	-0.18435064554905978
1104	9	-0.16034151292205934
1104	10-14	-0.1178064440218094
1104	15-19	-0.06962547200289038
1104	20-24	-0.05976616192376838
1104	25-29	-0.09794040466947251
1104	30-34	-0.08554592415701734
1104	35-39	-0.08480228094362019
1104	40-44	-0.061896605951119454
1104	45-49	-0.058772578908367734
1104	50-54	-0.08138377220133464
1104	55-59	-0.0907974304621817
1104	60-64	-0.12353911430620457
1104	65-69	-0.11008805260185284
1104	70-74	-0.11856990646313648
1104	75-79	-0.10942475269443008
1104	80-84	-0.07841827042524585
1104	85-89	-0.048751585968688005
1104	90-94	-0.04509995493946661
1104	95-99	-0.044177202955118844
1104	100-104	-0.03439533102106651
1104	105-109	-0.07369828240000231
1104	110-114	-0.059307269118889394
1104	115-119	-0.057066272728491185
1104	120-124	-0.10841888534214661
1104	125-129	-0.08951732756427333
1104	130-134	-0.1304274808302921
1104	135-139	-0.21533162080991275
1104	140-144	-0.11061934262388462
1104	145-149	-0.18715391962095396
1104	150	-0.23918640862584084
1105	1	-0.09321223211799179
1105	2	0.06617161275327277
1105	3	0.016640327212833483
1105	4	0.026283453083792097
1105	5	0.0010895090858582535
1105	6	-0.10880339022578767
1105	7	-0.02073302833679236
1105	8	-0.054227630700147245
1105	9	-0.11828500762513983
1105	10-14	-0.05809367818492461
1105	15-19	-0.062141594200952
1105	20-24	7.203972262317393E-4
1105	25-29	-0.012472682220817433
1105	30-34	0.0026068670926946425
1105	35-39	-0.005583645554665395
1105	40-44	-0.002675391378389236
1105	45-49	0.008100224622580754
1105	50-54	0.016675694835718957
1105	55-59	-0.009398296670184436
1105	60-64	0.009296812450052983
1105	65-69	0.023010703775845798
1105	70-74	0.014450613632000398
1105	75-79	-0.0642690861459343
1105	80-84	-0.0793559534959698
1105	85-89	-0.0855271231029846
1105	90-94	-0.0402125214215161
1105	95-99	-0.09263576232497428
1105	100-104	-0.0313979001914646
1105	105-109	-0.034917732708294125
1105	110-114	-0.018298590639233225
1105	115-119	-0.08612993929525459
1105	120-124	-0.0825390755492279
1105	125-129	-0.11875301607488353
1105	130-134	-0.05257198797494311
1105	135-139	-0.03087998605044362
1105	140-144	0.004353301663023501
1105	145-149	0.004519627559027128
1105	150	-0.049321708872284376
1106	1	0.08369900022646704
1106	2	0.06433430552886676
1106	3	-0.06345074494718972
1106	4	-0.10888063315810825
1106	5	-0.05309262221936706
1106	6	-0.13413338214780168
1106	7	-0.09249218150042537
1106	8	-0.11195285630960683
1106	9	-0.021219043008947835
1106	10-14	-0.12662461857154028
1106	15-19	-0.0553175348434678
1106	20-24	-0.0415886730030266
1106	25-29	-0.025535981258897777
1106	30-34	-0.030445780889003515
1106	35-39	-0.010267683268835981
1106	40-44	0.021451414583317785
1106	45-49	-0.006228991861725319
1106	50-54	-0.03220857007519129
1106	55-59	-0.011473339131065075
1106	60-64	0.01418052933103553
1106	65-69	-0.009938388360545503
1106	70-74	-0.002367063181949902
1106	75-79	0.03839691406757595
1106	80-84	-0.004160677387204714
1106	85-89	4.739287280841609E-5
1106	90-94	0.012307763687701367
1106	95-99	-0.0070235912770186815
1106	100-104	-0.041287979390226326
1106	105-109	0.021099859122330145
1106	110-114	0.005985497797588835
1106	115-119	0.007947232673402027
1106	120-124	0.015457080151065838
1106	125-129	0.027909159888807267
1106	130-134	0.028420846657034815
1106	135-139	0.07597402500618244
1106	140-144	-0.028051942891806902
1106	145-149	-3.4565398957653315E-4
1106	150	0.06408599425449069
1107	1	-0.014635627677066054
1107	2	-0.0523532591996414
1107	3	0.04907533139546416
1107	4	-0.12499507316402259
1107	5	-0.020424143154414764
1107	6	0.015083957695374295
1107	7	-0.02633428092341461
1107	8	0.034254607812307825
1107	9	0.044135669226584184
1107	10-14	-0.006523531132572202
1107	15-19	0.009280223700692147
1107	20-24	-0.008192978373642745
1107	25-29	8.508733053886886E-4
1107	30-34	-0.022637710168488923
1107	35-39	-0.021687271965390664
1107	40-44	-0.057658660871631184
1107	45-49	-0.05309468945013407
1107	50-54	-0.06278384008555093
1107	55-59	-0.03917403450020629
1107	60-64	-0.0544202622061718
1107	65-69	-0.0627440548792606
1107	70-74	-0.03494551413623981
1107	75-79	-0.059735760884329636
1107	80-84	-0.05113275165854958
1107	85-89	-0.01600623932129963
1107	90-94	-0.01189115627087034
1107	95-99	-0.011766711561087106
1107	100-104	-0.05812294018324593
1107	105-109	-0.028414603076221567
1107	110-114	-0.026113777243089942
1107	115-119	0.011792550028047799
1107	120-124	-0.026653217230197868
1107	125-129	-0.028793495829503968
1107	130-134	-0.050692557692720186
1107	135-139	-0.0026647899072642645
1107	140-144	-0.07401103447339352
1107	145-149	0.007696548999021502
1107	150	-0.05874412114719263
1108	1	-0.03619656635151358
1108	2	-0.015931596291210326
1108	3	0.029266503964770152
1108	4	-0.03809572753905144
1108	5	-0.007623760698741933
1108	6	0.05754405408304564
1108	7	0.05483215361697802
1108	8	0.0012031973688308994
1108	9	0.004871067378417138
1108	10-14	0.04217148810074889
1108	15-19	0.05093544985800946
1108	20-24	0.04123645523823427
1108	25-29	0.02393478142759875
1108	30-34	0.06626314980410086
1108	35-39	0.04775849891493067
1108	40-44	-0.00976039850261401
1108	45-49	0.0069190502147264965
1108	50-54	0.03933977268361133
1108	55-59	0.005238000694561151
1108	60-64	-0.022806730349607562
1108	65-69	0.031045152354238326
1108	70-74	-0.002016378972420796
1108	75-79	0.03694643613560089
1108	80-84	0.041600491035005405
1108	85-89	-0.017799160575222572
1108	90-94	-0.027086297094143674
1108	95-99	-0.027377037864312115
1108	100-104	0.024308527296959426
1108	105-109	0.007359361198439274
1108	110-114	0.03658052350413499
1108	115-119	0.05099662218615464
1108	120-124	0.08196775372085341
1108	125-129	0.1366216675431815
1108	130-134	0.134774679341362
1108	135-139	0.12373058746374
1108	140-144	0.23083498842972716
1108	145-149	0.17102206741437698
1108	150	0.2721066216568886
1109	1	-0.12051123154180488
1109	2	-0.22524577127693846
1109	3	-0.08442104541612849
1109	4	-0.04181874466160451
1109	5	-0.021372210787269807
1109	6	0.016919706487641406
1109	7	-0.039287179474136735
1109	8	-0.07490602020701687
1109	9	-0.013847422560850475
1109	10-14	0.003016323939888821
1109	15-19	0.018515730947072484
1109	20-24	-0.010258195764947686
1109	25-29	-0.00391603490717074
1109	30-34	0.007522917850835142
1109	35-39	0.012394853638483028
1109	40-44	0.018442788403731925
1109	45-49	0.03161666320687573
1109	50-54	0.022714952184990977
1109	55-59	-0.007256160104070375
1109	60-64	-0.029276542399408356
1109	65-69	-0.015152267439646039
1109	70-74	0.005391442385501932
1109	75-79	0.009678490323395295
1109	80-84	0.011380533365603185
1109	85-89	-0.014264693427570307
1109	90-94	0.032613674646995605
1109	95-99	0.02012096959833798
1109	100-104	0.008512325517237684
1109	105-109	0.06409868194793944
1109	110-114	0.07881858990666757
1109	115-119	0.06672733263673791
1109	120-124	0.009209101610373693
1109	125-129	-0.03012079051548966
1109	130-134	-0.025728789576781708
1109	135-139	-0.0534872536753781
1109	140-144	-0.06390716973910315
1109	145-149	-0.052135576604847245
1109	150	-0.015320208103716482
1110	1	0.0634512767136286
1110	2	-0.005615672521692261
1110	3	0.04340353086982418
1110	4	0.05545286809542205
1110	5	0.07537179059170285
1110	6	0.0639223617816711
1110	7	0.01890832453785407
1110	8	0.08838974003793965
1110	9	0.06490389201304936
1110	10-14	0.07291293597064907
1110	15-19	0.02244901741088512
1110	20-24	0.02985831567063002
1110	25-29	0.018692443828207672
1110	30-34	-0.004517552626204235
1110	35-39	0.02398373045491553
1110	40-44	0.0024310981376842733
1110	45-49	0.03827683786363423
1110	50-54	0.02086216122112461
1110	55-59	0.028186011233096053
1110	60-64	0.04105831516179137
1110	65-69	0.029408164224783206
1110	70-74	0.04034961036818885
1110	75-79	0.055492441559728434
1110	80-84	-0.022868014550731175
1110	85-89	0.05296052237558513
1110	90-94	0.039279148271276654
1110	95-99	0.014588638343141724
1110	100-104	0.07848711668639652
1110	105-109	0.0691781219152432
1110	110-114	0.06121731397107055
1110	115-119	0.06137669631518605
1110	120-124	0.1619479899505194
1110	125-129	0.07926806964484001
1110	130-134	0.06057353433809709
1110	135-139	0.0665107747058542
1110	140-144	0.07474284468974446
1110	145-149	0.07128699475013178
1110	150	0.10288095123910068
1111	1	-0.04983912367601562
1111	2	0.10150365408908613
1111	3	0.09575200068577061
1111	4	0.09977175092097923
1111	5	0.11843113035995145
1111	6	0.0649435930095521
1111	7	0.08313164456523481
1111	8	0.1033774925033697
1111	9	0.06339941053578002
1111	10-14	0.11927271050458188
1111	15-19	0.04011896828944117
1111	20-24	0.04446535823923625
1111	25-29	0.04759247766062202
1111	30-34	0.026026759200526328
1111	35-39	0.028731854362831655
1111	40-44	0.023475407080617572
1111	45-49	0.07904610157703473
1111	50-54	0.07558560107935364
1111	55-59	0.05503729962022419
1111	60-64	0.06081478305159749
1111	65-69	0.06913702868364169
1111	70-74	0.02803254568272706
1111	75-79	0.03300184242144866
1111	80-84	0.055981540614830294
1111	85-89	0.03140167095025248
1111	90-94	0.015256751377044964
1111	95-99	0.04257655880491029
1111	100-104	0.05047570864267925
1111	105-109	0.034634098222475984
1111	110-114	0.025871399196631728
1111	115-119	2.2774614506460011E-4
1111	120-124	0.0681492883450332
1111	125-129	0.054366054216409054
1111	130-134	0.09309433723053218
1111	135-139	0.07581818923480199
1111	140-144	0.1364176025461461
1111	145-149	0.10006264502420947
1111	150	0.1055676655509572
1112	1	-0.04520657931715277
1112	2	-0.08150679867844701
1112	3	-5.74124069338211E-4
1112	4	0.030952353258896892
1112	5	0.06716256111111107
1112	6	0.009571187048415197
1112	7	0.023123938965085244
1112	8	0.06989395048052671
1112	9	0.018694530888716088
1112	10-14	0.10481281659205166
1112	15-19	0.06568450867411713
1112	20-24	0.05421458029353943
1112	25-29	0.05190736883242408
1112	30-34	0.06500610546745378
1112	35-39	0.05598613656567153
1112	40-44	0.07803886278972527
1112	45-49	0.07286625311436978
1112	50-54	0.07029638161807839
1112	55-59	0.08883880097238261
1112	60-64	0.06768388015089499
1112	65-69	0.12153112987375181
1112	70-74	0.0963891481222916
1112	75-79	0.0898754474652037
1112	80-84	0.10720379054293261
1112	85-89	0.10516652095356704
1112	90-94	0.08396045788704498
1112	95-99	0.12284644155703006
1112	100-104	0.0988811438307593
1112	105-109	0.09171734947143051
1112	110-114	0.05849312020277608
1112	115-119	0.08551049375940067
1112	120-124	0.09262131926661965
1112	125-129	0.08748198063880608
1112	130-134	0.06998843543576072
1112	135-139	0.1179624951958047
1112	140-144	0.11355832893901407
1112	145-149	0.06421920555289518
1112	150	0.16986488313129655
1113	1	0.03785211018728063
1113	2	0.0031221532859504464
1113	3	-0.026769069481510144
1113	4	0.08683005261949006
1113	5	0.036031045591123245
1113	6	0.060054584785909526
1113	7	0.059045072310418334
1113	8	0.08017124637092365
1113	9	0.10500793220547422
1113	10-14	0.04555942274212299
1113	15-19	0.04282763494953201
1113	20-24	0.01019208782080483
1113	25-29	0.03508053713314041
1113	30-34	-0.0014180809136377093
1113	35-39	-0.005939597906305494
1113	40-44	0.02280385704725063
1113	45-49	0.007143406791833229
1113	50-54	0.04436431040494426
1113	55-59	0.0017857709921145215
1113	60-64	0.021358000435427016
1113	65-69	0.01558943304833349
1113	70-74	0.04607082366626969
1113	75-79	0.05665576313399612
1113	80-84	0.06468274009174024
1113	85-89	0.079499550936454
1113	90-94	0.06886471128400729
1113	95-99	0.0798188985240813
1113	100-104	0.10961049714986615
1113	105-109	0.062011226813879716
1113	110-114	0.12086086929821249
1113	115-119	0.12599437986192186
1113	120-124	0.1441515302189913
1113	125-129	0.09935600384726939
1113	130-134	0.0950058570134047
1113	135-139	0.12181237933128841
1113	140-144	0.07969789264242877
1113	145-149	0.04373930836602824
1113	150	0.05711587969604892
1114	1	-0.05401450528564311
1114	2	-0.0419676134015603
1114	3	0.04002499298599105
1114	4	0.13295613946993257
1114	5	0.06676212306103935
1114	6	0.09275175239362454
1114	7	0.16046563447421391
1114	8	0.14879846004016883
1114	9	0.14210380233431152
1114	10-14	0.12422223874898464
1114	15-19	0.07607167886143884
1114	20-24	0.0443560910002887
1114	25-29	0.07074018520612668
1114	30-34	0.06248172017556186
1114	35-39	0.05451690175147661
1114	40-44	0.04388110224174113
1114	45-49	0.03626331957412532
1114	50-54	0.03787760943504992
1114	55-59	0.03910740938022883
1114	60-64	0.0649814580419914
1114	65-69	0.010817422842116287
1114	70-74	-6.438046450085722E-5
1114	75-79	0.016477578089151734
1114	80-84	0.032691134410264056
1114	85-89	0.020643788879489477
1114	90-94	0.030465583717848688
1114	95-99	0.0643902258331579
1114	100-104	0.012022293883305224
1114	105-109	0.032052260995975246
1114	110-114	0.0028360253513781686
1114	115-119	0.007004901973893141
1114	120-124	-0.007581305216660894
1114	125-129	0.04465137162679156
1114	130-134	0.031976647045006246
1114	135-139	0.16809282430592987
1114	140-144	0.19146132875276578
1114	145-149	0.1289623414412091
1114	150	0.03902026125551572
1115	1	0.14957327166113998
1115	2	0.10601359903790097
1115	3	-0.07249337288875779
1115	4	0.12451990419577896
1115	5	0.042844317312322744
1115	6	0.08384397162780033
1115	7	0.1035677566941402
1115	8	0.16704936573637497
1115	9	0.12729745954354854
1115	10-14	0.08730118151110844
1115	15-19	0.05263576184071184
1115	20-24	0.03841673949469282
1115	25-29	0.0023455615673171337
1115	30-34	0.06409417767546444
1115	35-39	0.03342070434948852
1115	40-44	0.05615011655783775
1115	45-49	0.037304909992570856
1115	50-54	0.04777842045085379
1115	55-59	0.1155528899163798
1115	60-64	0.07781754691956166
1115	65-69	0.06829791655925987
1115	70-74	0.1073985297509168
1115	75-79	0.06633834221563717
1115	80-84	0.1327085057430466
1115	85-89	0.11740319989849723
1115	90-94	0.09257923732021567
1115	95-99	0.09295246294256998
1115	100-104	0.050152051108987905
1115	105-109	0.03660951743928109
1115	110-114	0.052792875491142865
1115	115-119	0.042459739160747745
1115	120-124	0.10473706490146384
1115	125-129	0.12175309072571139
1115	130-134	0.10909061890079386
1115	135-139	0.1264044368429893
1115	140-144	0.0856920306607023
1115	145-149	0.1470015820885422
1115	150	0.26505551312657616
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	15.0
15	48.0
16	121.0
17	215.0
18	438.0
19	704.0
20	910.0
21	1184.0
22	1507.0
23	1834.0
24	2172.0
25	2536.0
26	2917.0
27	3521.0
28	4086.0
29	4739.0
30	5813.0
31	9133.0
32	12986.0
33	15449.0
34	21665.0
35	29731.0
36	26006.0
37	39910.0
38	87899.0
39	562060.0
40	162400.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.3049	36.125299999999996	7.8532	18.7166
2	21.806047903891375	35.8883078203579	21.350875697574722	20.954768578176004
3	30.220744768942165	27.82309504380669	21.91538970339523	20.040770483855912
4	25.0536	26.4492	18.3836	30.1136
5	22.8741	37.8123	22.48	16.8336
6	37.147200000000005	26.070999999999998	20.1103	16.6715
7	24.341099999999997	23.1221	37.033500000000004	15.503300000000001
8	33.2996	20.9758	25.677	20.0476
9	31.839699999999997	26.0401	23.674400000000002	18.445800000000002
10-14	20.24002	31.744220000000002	24.77148	23.24428
15-19	25.883260000000003	31.086399999999998	18.9468	24.08354
20-24	30.416559999999997	28.977140000000002	23.45814	17.14816
25-29	29.808960000000003	29.81146	23.08868	17.2909
30-34	27.66832	30.061139999999998	22.60192	19.66862
35-39	25.294719999999998	30.000680000000003	23.7758	20.9288
40-44	24.89692	30.23364	23.88018	20.98926
45-49	24.57744	30.028660000000002	24.10384	21.29006
50-54	24.59608	30.163240000000002	23.62204	21.61864
55-59	24.260080000000002	30.17058	23.7961	21.773239999999998
60-64	24.6522	30.50574	23.99728	20.84478
65-69	24.44046	30.781920000000003	23.74552	21.0321
70-74	24.45292	30.97728	23.5628	21.007
75-79	24.57804	31.21356	23.36228	20.846120000000003
80-84	24.73572	31.19002	23.016280000000002	21.05798
85-89	25.03342	31.691039999999997	22.67274	20.6028
90-94	24.30502	31.73802	22.61716	21.3398
95-99	24.516579999999998	31.25208	23.07508	21.15626
100-104	23.99658	31.886680000000002	22.953879999999998	21.16286
105-109	24.45424	31.633499999999998	22.69258	21.21968
110-114	24.39218	31.621719999999996	22.60852	21.37758
115-119	24.800240000000002	31.46254	22.7636	20.97362
120-124	25.27614	31.11902	22.75312	20.85172
125-129	26.18953189928502	30.716698156849308	22.428130083154482	20.66563986071119
130-134	26.343159999999997	30.37364	22.52274	20.76046
135-139	25.820880000000002	30.22556	23.0846	20.86896
140-144	26.179160000000003	29.953000000000003	23.07518	20.792659999999998
145-149	26.9484	30.09788	22.20954	20.74418
150	27.7874	29.744	21.8537	20.6149
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	187.0
1	276.0
2	419.0
3	448.0
4	412.5
5	383.0
6	355.5
7	351.5
8	338.0
9	323.5
10	342.5
11	343.5
12	487.0
13	525.5
14	474.0
15	846.0
16	1107.0
17	1024.0
18	1006.0
19	1092.5
20	1322.5
21	1726.5
22	2353.5
23	3207.0
24	4235.0
25	5420.5
26	6869.0
27	8627.5
28	10329.0
29	11831.5
30	13427.0
31	15115.5
32	16912.5
33	18530.0
34	20177.5
35	21778.0
36	23033.0
37	24379.0
38	25672.0
39	26515.0
40	27332.5
41	28047.0
42	28306.0
43	29123.5
44	30027.5
45	30207.5
46	31099.0
47	32471.0
48	33098.0
49	34617.0
50	35923.5
51	36645.0
52	37912.5
53	37354.5
54	33572.0
55	29123.0
56	25927.0
57	23846.5
58	22337.0
59	19790.0
60	16327.0
61	14062.5
62	11790.0
63	8956.5
64	7041.5
65	5888.5
66	5450.5
67	4955.5
68	4329.0
69	3921.0
70	3745.0
71	3494.0
72	3489.0
73	3422.0
74	3437.5
75	2970.0
76	2194.0
77	2093.0
78	2276.0
79	2387.5
80	1794.0
81	1173.0
82	954.0
83	915.5
84	856.0
85	670.5
86	546.0
87	448.5
88	350.0
89	339.5
90	295.0
91	229.5
92	159.0
93	53.5
94	28.0
95	22.0
96	14.0
97	12.0
98	16.0
99	13.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.2795
3	0.3691
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	5.8E-4
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	1000000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.38985959681902
#Duplication Level	Percentage of total
1	58.64417800816404
2	9.896818894108344
3	3.233487416401395
4	1.652938091766708
5	1.1645000256235807
6	0.8897406587239933
7	0.7407228786754397
8	0.6699996123010555
9	0.5969562994624656
>10	11.550671504672334
>50	4.636492374977216
>100	5.402692386096333
>500	0.5717477355699466
>1k	0.34905411345709153
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTGAATGGGGAATCAGGGTTCGATTCCGG	1706	0.1706	Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGCGCGACCTCAGATCAGACGT	1421	0.1421	Clontech Universal Primer Mix Long (100% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0011	0.0	0.0	0.0	0.0374	0.0
2	0.002	0.0	0.0	0.0	0.1253	0.0
3	0.0032	0.0	0.0	0.0	0.2184	0.0024
4	0.0049	0.0	0.0	0.0	0.2983	0.0026
5	0.0063	0.0	0.0	0.0	0.3765	0.0031
6	0.0082	0.0	0.0	0.0	0.4503	0.0035
7	0.0091	0.0	0.0	0.0	0.5181	0.0039
8	0.011	0.0	0.0	0.0	0.5836	0.0043
9	0.0128	0.0	0.0	0.0	0.6586	0.0053
10-11	0.016550000000000002	0.0	0.0	5.0E-5	0.77295	0.006
12-13	0.0216	0.0	0.0	1.0E-4	0.917	0.006999999999999999
14-15	0.0271	0.0	0.0	1.0E-4	1.0749	0.00835
16-17	0.03465	0.0	0.0	1.0E-4	1.2347000000000001	0.009649999999999999
18-19	0.04965	0.0	0.0	2.0E-4	1.4132	0.0104
20-21	0.07039999999999999	0.0	0.0	2.0E-4	1.5937000000000001	0.011300000000000001
22-23	0.13155	0.0	0.0	2.0E-4	1.77705	0.01225
24-25	0.2032	0.0	0.0	2.0E-4	2.0129	0.0132
26-27	0.2164	0.0	0.0	2.5E-4	2.2531	0.0138
28-29	0.2326	5.0E-5	0.0	3.0E-4	2.5105500000000003	0.0149
30-31	0.25455	1.0E-4	0.0	3.0E-4	2.95665	0.016100000000000003
32-33	0.2832	1.0E-4	0.0	3.0E-4	3.46765	0.0171
34-35	0.3182	1.0E-4	0.0	3.0E-4	3.794	0.0179
36-37	0.3629	1.0E-4	0.0	3.0E-4	4.11285	0.019549999999999998
38-39	0.4175	1.0E-4	0.0	3.0E-4	4.4437999999999995	0.0213
40-41	0.4656	1.0E-4	0.0	3.0E-4	4.79065	0.0233
42-43	0.5153	3.0E-4	0.0	3.0E-4	5.1629000000000005	0.0267
44-45	0.5432	3.0E-4	0.0	3.0E-4	5.4928	0.03
46-47	0.6076999999999999	3.0E-4	0.0	3.0E-4	5.8102	0.03265
48-49	0.64775	3.0E-4	0.0	3.0E-4	6.1471	0.03335
50-51	0.69075	3.0E-4	0.0	3.0E-4	6.4956	0.0346
52-53	0.7321500000000001	3.0E-4	0.0	3.0E-4	6.8420000000000005	0.03595
54-55	0.78355	3.0E-4	0.0	3.0E-4	7.20445	0.037
56-57	0.85895	3.0E-4	0.0	3.0E-4	7.5881	0.038
58-59	0.9498	3.0E-4	0.0	3.0E-4	7.9527	0.038650000000000004
60-61	1.0908	3.0E-4	0.0	3.0E-4	8.31455	0.0402
62-63	1.2149	3.0E-4	0.0	3.0E-4	8.68225	0.0416
64-65	1.3308	3.0E-4	0.0	3.0E-4	9.0671	0.043649999999999994
66-67	1.47525	3.0E-4	0.0	3.0E-4	9.471699999999998	0.04735
68-69	1.7190500000000002	3.0E-4	0.0	3.0E-4	9.86815	0.05555
70-71	1.9943	3.0E-4	0.0	3.0E-4	10.2837	0.06609999999999999
72-73	2.1323	3.0E-4	0.0	3.0E-4	10.711649999999999	0.07105
74-75	2.3859500000000002	3.0E-4	0.0	3.0E-4	11.1424	0.07555
76-77	2.8714500000000003	3.0E-4	0.0	3.0E-4	11.5823	0.08215
78-79	3.73285	3.0E-4	0.0	3.0E-4	12.0293	0.08915000000000001
80-81	4.36815	3.0E-4	0.0	3.0E-4	12.504750000000001	0.0987
82-83	4.70815	3.0E-4	0.0	3.0E-4	12.9893	0.10869999999999999
84-85	4.96455	3.0E-4	0.0	3.0E-4	13.4875	0.12135
86-87	5.242850000000001	3.0E-4	0.0	3.0E-4	14.0098	0.13505
88-89	5.52935	3.0E-4	0.0	6.0E-4	14.57245	0.1524
90-91	5.83625	3.0E-4	0.0	6.5E-4	15.1271	0.17665
92-93	6.1625	3.0E-4	0.0	7.0E-4	15.6482	0.21400000000000002
94-95	6.4786	3.0E-4	0.0	7.0E-4	16.19185	0.2788
96-97	6.76605	3.0E-4	0.0	7.0E-4	16.73435	0.34115
98-99	7.072150000000001	3.0E-4	0.0	7.5E-4	17.2961	0.36875
100-101	7.40685	3.0E-4	0.0	8.0E-4	17.8637	0.39695
102-103	7.80445	3.0E-4	0.0	8.0E-4	18.46335	0.43335
104-105	8.271899999999999	3.0E-4	0.0	8.0E-4	19.087000000000003	0.47285
106-107	8.689	3.0E-4	0.0	8.0E-4	19.68575	0.5206
108-109	9.069849999999999	3.0E-4	0.0	8.0E-4	20.285	0.5751
110-111	9.46755	3.0E-4	0.0	8.0E-4	20.85695	0.62875
112-113	9.88425	3.0E-4	0.0	8.0E-4	21.4558	0.6851
114-115	10.3307	3.0E-4	0.0	9.0E-4	22.0398	0.7474000000000001
116-117	10.78845	3.0E-4	0.0	9.0E-4	22.645000000000003	0.8117000000000001
118-119	11.22195	3.0E-4	0.0	9.0E-4	23.2604	0.86775
120-121	11.6912	3.0E-4	0.0	9.0E-4	23.86785	0.929
122-123	12.18995	3.0E-4	0.0	9.0E-4	24.4845	1.00865
124-125	12.677700000000002	3.0E-4	0.0	9.0E-4	25.077550000000002	1.11695
126-127	13.17025	3.0E-4	0.0	9.0E-4	25.6678	1.22105
128-129	13.68315	3.0E-4	0.0	9.0E-4	26.2731	1.3193
130-131	14.21195	3.0E-4	0.0	9.0E-4	26.8461	1.4335
132-133	14.73845	3.0E-4	0.0	9.0E-4	27.4331	1.58
134-135	15.28195	3.0E-4	0.0	9.0E-4	28.054250000000003	1.7226
136-137	15.84155	3.0E-4	0.0	9.0E-4	28.73475	1.873
138-139	16.41065	3.0E-4	0.0	9.0E-4	29.3268	2.05605
>>END_MODULE
